生物芯片与疾病诊断

§1.遗传病和传染病诊断

遗传性疾病的产前诊断或早期准确诊断对于早期干预和预防治疗具有重要意义。耳聋是我国第一大残疾人群,我们研制成功了国际上第一款经过政府批准可用于临床诊断的遗传性耳聋基因检测芯片。传染性疾病是危害人类健康的重大疾病,其中分枝杆菌的早期鉴别诊断,结核耐药的快速诊断,乙肝耐药的快速诊断工作具有重要的临床意义。这些传染病疾病诊断芯片已陆续获得医疗器械批文。 

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代表论文

  1. Zhu, L., Jiang, G., Wang, S., Wang, C., Li, Q., Yu, H., Zhou, Y., Zhao, B., Huang, H., Xing, W., Mitchelson, K., Cheng, J. Zhao, Y. and Guo, Y.(2010)"Biochip system for rapid and accurate identification of mycobacteria species from isolates and sputum"
    Journal of Clinical Microbiology,48 (10):3654-3660[摘要]
  2. Guo, Y., Zhou, Y., Wang, C., Zhu, L., Wang, S., Li, Q., Jiang, G., Zhao, B., Huang, H., Yu, H., Xing W., Mitchelson, K., Cheng, J. and Zhao, Y.(2009)"Rapid, accurate determination of Multidrug resistance in M. tuberculosis isolates and sputum using a biochip system"
    International Journal of Tuberculosis and Lung Disease,13(7):914-920[摘要]
  3. Zhu, L., Zhang, Z., Liang, D., Jiang, D., Wang, C., Du, N., Zhang, Q., Mitchelson, K. and Cheng, J.(2007)"Multiplex asymmetric PCR (MAPCR)-based oligonucleotide microarray for detection of drug resistance genes containing single mutations in Enterobacteriaceae"
    Antimicrobial Agents?and Chemotherapy,51(10):3707-3713[摘要]
  4. Zhu, L., Zhang, Z., Wang, C., Yang, H., Jiang, D., Zhang, Q., Mitchelson, K. and Cheng, J.(2007)"Simultaneous detection of antibiotic resistance genes among staphylococcal clinical isolates with a DNA microarray"
    Journal of Clinical Microbiology,45(11):3514-3521[摘要]
  5. Zhou, Y., Yang, R., Tao, S., Li, Z., Zhang, Q., Gao, H., Zhang, Z., Du, J., Zhu, P., Ren, L., Zhang, L., Wang, D., Guo, L., Wang, Y., Guo, Y., Zhang, Y., Zhao, C., Wang, C., Jiang, D., Liu, Y., Yang, H., Rong, L., Zhao, Y., An, S., Li, Z., Fan, X., Wang, J., Cheng, Y., Liu, O., Zheng, Z., Zuo, H., Shan, Q., Ruan, L., Lv, Z., Hong, T. and Cheng, J.(2005)"The design and application of DNA chips for early detection of SARS-CoV from clinical samples"
    Journal of Clinical Virology,33(2):123-131[摘要]

§2.疾病诊断

研发了基于微阵列、微流体和微液滴等微加工技术,整合光机电软等工程手段的一批诊断技术平台。

图5 用于基因快速分型的微流控芯片及检测控制仪器 

  图5 用于基因快速分型的微流控芯片及检测控制仪器  

图6 恒温扩增微流控核酸分析仪和蝶式芯片

图6 恒温扩增微流控核酸分析仪和蝶式芯片

图7 微液滴技术

图7 微液滴技术 

代表论文

  1. Zhuang, B., Gan, W., Wang, S., Han, J., Xiang, G., Li, C., Sun, J. and Liu, P. (2015) "Fully automated sample preparation microsystem for genetic testing of hereditary hearing loss using two-color multiplex allele-specific PCR" Analytical Chemistry, 87:1202-1209.
  2. Gan, W., Zhuang, B., Zhang, P., Han, J., Li, C. and Liu, P. (2014) "A filter paper-based microdevice for low-cost, rapid, and automated DNA extraction and amplification from diverse sample types" Lab on a Chip, 14:3719-3728. (Cover paper)